Show simple item record

dc.contributor.authorJain, A.
dc.contributor.authorBhosle, N.B.
dc.date.accessioned2008-07-24T09:31:25Z
dc.date.available2008-07-24T09:31:25Z
dc.date.issued2008
dc.identifier.citationBiofouling, vol.24(4); 283-290p.
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/1321
dc.description.abstractPseudomonas sp CE-2 cells attach and form biofilms on 304-stainless steel (SS) coupons. A series of experiments were carried out in order to understand the role of exopolysaccharides (EPS) in the formation and maintenance of CE-2 biofilms on SS coupons. The biofilm density and EPS concentration increased over the period of incubation and the highest values for both were recorded after 72 h. Calcofluor and the lectin concanavalin A (Con A) showed a positive interaction with 72-h old biofilms, indicating the presence of beta 1-4 linked polymers, and alpha-D-glucose and alpha-D-mannose in the biofilm matrix of CE-2. When the CE-2 cells were grown in the presence of calcofluor (200 mu g ml sup(-1)), biofilm formation was significantly reduced (approx. 85%). Conversely, the lectins Con A or WGA did not influence the CE-2 biofilms on the SS coupons. Furthermore, treatment with cellulase, an enzyme specific for the degradation of beta 1-4 linked polymers, removed substantial amounts of CE-2 biofilm from SS coupons. These results strongly suggest the involvement of beta 1-4 linked polymers in the formation and maintenance of Pseudomonas sp. CE-2 biofilms on SS coupons.
dc.language.isoen
dc.publisherTaylor and Francis
dc.rightsThis is a preprint of an article whose final and definitive form has been published in the "Biofouling" © 2008 Taylor & Francis; "Biofouling" is available online at http://www.informaworld.com/ with open URL of artilce : http://www.informaworld.com/openurl?genre=article&issn=0892-7014&volume=24&spage=183
dc.subjectPseudomonas sp.
dc.subjectb 1-4 linked polymer
dc.subjectbiofilms
dc.subjectcalcofluor
dc.titleRole of beta 1-4 linked polymers in the biofilm structure of marine Pseudomonas sp. CE-2 on 304 stainless steel coupons
dc.typeJournal Article


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record