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    Opisthorchis viverrini: Detection by polymerase chain reaction (PCR) in human stool samples

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    Exp_Parasitol_120_353.pdf (166.7Kb)
    Date
    2008
    Author
    Umesha, K.R.
    SanathKumar
    Parvathi, A.
    Duenngai, K.
    Sithithaworn, P.
    Karunasagar, Indrani
    Karunasagar, Iddya
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    Abstract
    A polymerase chain reaction (PCR) assay was evaluated for detection of Opisthorchis viverrini eggs in the stool specimens of light and heavily infected individuals in Khon Kaen province of Thailand. A total of 75 fecal specimens were analyzed by PCR following DNA extraction. All the microscopically positive samples were positive by PCR, while 23 of 30 (76.6%) microscopically negative samples were also PCR positive. The sensitivity of the assay was 5 eggs/g of stool. This method is potentially useful in the diagnosis of human opisthorchiasis in endemic areas for treatment and in epidemiological investigations.
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    http://drs.nio.org/drs/handle/2264/1495
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