Show simple item record

dc.contributor.authorAnil, A.C.
dc.contributor.authorTulaskar, A.S.
dc.contributor.authorKhandeparker, D.C.
dc.contributor.authorWagh, A.B.
dc.date.accessioned2009-01-12T08:40:14Z
dc.date.available2009-01-12T08:40:14Z
dc.date.issued1997
dc.identifier.citationCryobiology, Vol.34; 131-140p.
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/1984
dc.description.abstractThe possibility of cryopreserving B. amphitrite (Cirripedia; thoracica) nauplii is explored. The effects on the postthaw survival of a range of cryobiological variables are reported; three different cryoprotectants (ethylene glycol, EG; dimethyl sulfoxide, Me sub(2)SO; and glycerol, GL), the influence of the final temperature (-20, -30, -40, and -50 degrees C), and the effect of transferring to liquid nitrogen (-196 degrees C) were studied. The larvae showed increasing susceptibility to cryoprotectant concentration. Equilibration of larvae for 15 min at 5 degrees C with each of the cryoprotectants revealed and GL was the least toxic. EG at 3 to 4 M did not cause marked damage as the equilibration period was extended up to 2 h. This was not true with Me sub(2)SO and GL. The postthaw survival rate decreased as the final temperature was reduced, and none of the larvae revived after thawing from -50 degrees C. The postthaw survival with 2 mol/liter EG as the cryoprotectant ranged from 86% after cooling to -20 degrees C to 56% after cooling to -40 degrees C. The larvae that were transferred to liquid nitrogen from different temperatures revealed that the greatest survival (36%) was achieved when the larvae were transferred from -40 to -196 degrees C. When the larvae reared after cooling to -40 degrees C and then transferring to liquid nitrogen, the rate of subsequent metamorphosis was 30% in the case of EG at 3M. Me sub(2)SO and GL gave results that were inferior in comparison to those obtained with EG
dc.language.isoen
dc.publisherAcademic Press
dc.rightsCopyright [1997]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subjectcrustacean larvae
dc.subjectnauplii
dc.subjectsurvival
dc.subjectfreezing storage
dc.subjectcryobiology
dc.subjecttemperature tolerance
dc.subjectcooling
dc.subjectrearing
dc.subjectmetamorphosis
dc.subjectBalanus amphitrite
dc.titleCryoperservation of Balanus amphitrite nauplii
dc.typeJournal Article


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record