Biological investigations of Indian phaeophyceae. 15, effect of antibacterial substance extracted from Sargassum johnstionii Setchell et Gardner collected from Okha, west coast of India on the growth of bacteria
Abstract
Cruide either extract and two fractions from the lipid namely fraction A (unsaponifiable) and fraction B (saponifiable) were obtained from a dry sample of Sargassum johnstionii. From these extracts different concentrations viz., 150 mu g, 200 mu g, 250 mu g and 300 mu g of extracts (added to 0.2ml solvent) were made and used per disc to test their efficiency to inhibit the growth of Staphylococcs aureus, S. albus, Bacillus magatherium (gram-positive), Proteus vulgaris, Escherichia coli and Psuedomonas aeruginosa (gram-negative). 250 mu g concentrations showed best antibacterial activity from all the extract. Highest antibacterials activity was exhibited by crude ether extract. Fraction B showed better activity than fraction A. Gram-positive bacteria were more sensitive to fractions as well as crule extracts than gram-negative bacteria. Highest antibacterial activity by crude ether extract was observed against Proteus vulgaris
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