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    A simple method to assess bacterial attachment to surfaces

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    Biofouling_9_31.pdf (822.2Kb)
    Date
    1995
    Author
    Sonak, S.
    Bhosle, N.B.
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    Abstract
    The crystal violet microplate adhension assay was modified to evaluate bacterial adhesion to metal and non-metal surfaces. Both viable cell count and the absorbance of the crystal violet stained cells attached to aluminium increased over the period of ineubation. There was a highly significant positive linear relationship between crystal violet stained attached cells and the viable cell count of cells attached to aluminium panels (r = 0.9997; p less than 0.001: n = 6). The method is relatively simple, sensitive, less time consuming and therefore many samples can be analysed in a short period of time. When the calibrated method was employed to assess the attachment of Vibrio sp to polystyrene, stainless steel and copper, it give a fairly reliable estimate of bacterial adhension to these surfaces
    URI
    http://drs.nio.org/drs/handle/2264/2312
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