A simple method to assess bacterial attachment to surfaces
Abstract
The crystal violet microplate adhension assay was modified to evaluate bacterial adhesion to metal and non-metal surfaces. Both viable cell count and the absorbance of the crystal violet stained cells attached to aluminium increased over the period of ineubation. There was a highly significant positive linear relationship between crystal violet stained attached cells and the viable cell count of cells attached to aluminium panels (r = 0.9997; p less than 0.001: n = 6). The method is relatively simple, sensitive, less time consuming and therefore many samples can be analysed in a short period of time. When the calibrated method was employed to assess the attachment of Vibrio sp to polystyrene, stainless steel and copper, it give a fairly reliable estimate of bacterial adhension to these surfaces
Collections
- Scholarly literature [9069]