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dc.contributor.authorParvathi, A.
dc.contributor.authorGeorge, J.
dc.contributor.authorKumar, S.
dc.date.accessioned2009-06-26T04:15:03Z
dc.date.available2009-06-26T04:15:03Z
dc.date.issued2009
dc.identifier.citationCurrent Microbiology, vol.59; 42-47
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/3366
dc.description.abstractVibrio harveyi, pathogenic to fish, harbor a hemolysin gene vhh, the homologues of which are found in many species of the Genus Vibrio. It is investigated that the prevalence of vhh gene among V. harveyi isolated from Penaeus monodon hatcheries in India by polymerase chain reaction (PCR). The vhh was detected in 67 of the 70 V. harveyi isolates tested in this study using different combinations of PCR primers. A variant vhh gene detected in a minority of strains was cloned, sequenced, and the recombinant protein was expressed in Escherichia coli. The deduced amino acid sequence of the cloned gene was 86% similar to the previously reported amino acid sequences of VHH. The results of this study suggest that though V. harveyi strains invariably harbor vhh, the sequence variants of the hemolysin gene exist that may impede their detection by PCR.
dc.language.isoen
dc.publisherSpringer
dc.rightsAn edited version of this paper was published by Springer. This paper is for R & D purpose and Copyright [2009] Springer.
dc.subjectbacterial diseases
dc.subjectpolymerase chain reaction
dc.subjectVibrio harveyi
dc.subjectmicroorganisms
dc.titlePrevalence and heterogeneity of Hemolysin gene vhh among hatchery isolates of Vibrio harveyi in India
dc.typeJournal Article


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