Show simple item record

dc.contributor.authorDeSouza-Ticlo, D.
dc.contributor.authorGarg, S.
dc.contributor.authorRaghukumar, C.
dc.date.accessioned2009-12-08T07:14:50Z
dc.date.available2009-12-08T07:14:50Z
dc.date.issued2009
dc.identifier.citationMarine Drugs, vol.7(4); 672-688
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/3466
dc.description.abstractThe effects of various synthetic medium components and their interactions with each other ultimately impact laccase production in fungi. This was studied using a laccase-hyper-producing marine-derived basidiomycete, Cerrena unicolor MTCC 5159. Inducible laccases were produced in the idiophase only after addition of an inducer such as CuSO sub(4). Concentration of carbon and nitrogen acted antagonistically with respect to laccase production. A combination of low nitrogen and high carbon concentration favored both biomass and laccase production. The most favorable combination resulted in 917 U L sup(-1) of laccase. After sufficient growth had occurred, addition of a surfactant such as Tween 80 positively impacted biomass and increased the laccase activity to around 1,300 U L sup(-1). Increasing the surface to volume ratio of the culture vessel further increased its activity to almost 2,000 U L sup(-1)
dc.language.isoen
dc.publisherMolecular Diversity Preservation International
dc.subjectmarine-derived basidiomycete
dc.subjectlaccase
dc.subjectPlackett Burman Design
dc.subjectCentral Composite Design
dc.titleEffects and interactions of medium components on laccase from a marine-derived fungus using response surface methodology
dc.typeJournal Article


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record