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dc.contributor.authorWahidullah, S.
dc.contributor.authorRajamanickam, Y.R.
dc.date.accessioned2010-02-16T07:40:46Z
dc.date.available2010-02-16T07:40:46Z
dc.date.issued2010
dc.identifier.citationEnvironmental Science and Pollution Research, vol.17(2); 441-452
dc.identifier.otherno
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/3546
dc.description.abstractMutagenic nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) have been known to arise in the environment through direct emissions from combustion sources and nitration of PAHs, primarily in the atmosphere. In the marine environment, PAHs are one of the classic anthropogenic organic pollutants, while nitrite (NO sub(2) sup(-)) is produced naturally via various biological processes like imbalance in ditrification/denitrification or eutrophication and subsequent oxygen depletion from an oversupply of nutrients. In this paper, we report the formation of PAH-DNA adducts in fish contaminated with PAHs and exposed to NO sub(2) sup (-) in the ambient water. Electrospray ionization tandem mass spectrometric (ESI-MS/MS) analysis of the bile of the euryhaline fish Oreochromis mossambicus exposed simultaneously to field relevant sublethal concentrations of phenanthrene and NO sub(2) sup(-) and collision-induced dissociation of selected ions revealed the presence of DNA-PAH adducts. The present study indicates that, although several high sensitivity techniques have been developed for the analysis of PAH derived DNA adducts, MS/MS has emerged as a powerful tool in the detection and structure elucidation of DNA adducts.
dc.language.isoen
dc.publisherSpringer-Verlag
dc.relation.requiresdnadetection.jpg
dc.rightsCopyright [2009] Springer. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subjectbrackishwater fish
dc.subjectDNA damage
dc.subjectaromatic hydrocarbons
dc.subjectOreochromis mossambicus
dc.titleDetection of DNA damage in fish Oreochromis mossambicus induced by co-exposure to phenanthrene and nitrite by ESI-MS/MS
dc.typeJournal Article


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