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dc.contributor.authorRavindran, C.
dc.contributor.authorNaveenan, T.
dc.contributor.authorVaratharajan, G.R.
dc.date.accessioned2010-06-21T07:39:05Z
dc.date.available2010-06-21T07:39:05Z
dc.date.issued2010
dc.identifier.citationBotanica Marina, vol.53(3); 275-282
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/3637
dc.description.abstractAlkaline cellulases were screened from 54 fungal isolates obtained from mangrove leaves and mangrove wood litters as endophytes and wood litter fungi, respectively. Only three fungal isolates tested positive for alkaline cellulases under plate assay, among which Chaetomium sp. (NIOCC 36) was found to grow in a wide range of pH (between 4 and 12). This alkaline tolerant fungus was further tested for production of alkaline cellulases (Beta-endoglucanase, Beta-exoglucanase, Beta-glucosidase) using agricultural and industrial wastes as substrates in submerged fermentation (SMF) and solid state fermentation (SSF) processes. Cotton seed under SSF conditions had maximum enzyme production at high alkaline pH. Cellulase enzymes produced under alkaline cotton seed SSF conditions at 50 degrees C had higher stability and activity than under non-alkaline conditions (range tested: pH 5, 7, 12). The agricultural and industrial wastes used as carbon sources were economical for commercial production of cellulose. The high specific activity, and stability of cellulases under neutral to alkaline pH and high temperature will be of use in various industrial and biotechnological applications.
dc.language.isoen
dc.publisherWalter de Gruyter
dc.rightsAn edited version of this paper was published by Walter de Gruyter. Copyright [2010] Walter de Gruyter. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subjectagro wastes
dc.subjectalkaline cellulase
dc.subjectmarine fungi
dc.titleOptimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates
dc.typeJournal Article


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