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dc.contributor.authorNayak, S.
dc.contributor.authorSingh, SanjayKumar
dc.contributor.authorRamaiah, N.
dc.contributor.authorSreepada, R.A.
dc.date.accessioned2010-07-15T12:24:17Z
dc.date.available2010-07-15T12:24:17Z
dc.date.issued2010
dc.identifier.citationFish & Shellfish Immunology, vol.29(3); 544-549
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/3656
dc.description.abstractA subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi. As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 hypothetical proteins were identified, a few being first reports from shrimps. The most abundant immune relevant genes were ferritin, hemocyanin, and TCTP (translationally controlled tumor protein) indicating their upregulation as also confirmed through qPCR. Post-infection qPCR analyses confirmed 2.04, 2.09, 3.28, 5.49, 6.47, and 11.63 fold rise respectively in ferritin, penaeidin, MnSOD, lysozyme, TCTP, and hemocyanin genes. These genes may be involved in the regulation of the host defense against V. harveyi
dc.language.isoen
dc.publisherElsevier
dc.rightsAn edited version of this paper was published by Elsevier. Copyright [2010] Elsevier
dc.subjectPenaeus monodon
dc.subjectShrimp diseases
dc.subjectVibrio harveyi
dc.titleIdentification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae
dc.typeJournal Article


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