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    Role of 12 S mitochondrial gene on dimorphism and coiling direction in benthic foraminiferal species Pararotalia nipponica

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    Gondwana_Geol_Magz_6_19.pdf (53.38Kb)
    Date
    2003
    Author
    Saraswat, R.
    Mazumder, A.
    Kurtarkar, S.R.
    Nigam, R.
    Ganguly, A.
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    Abstract
    Despite the extensive use of foraminiferal proxies for paleoclimatic reconstructions, the question how and why morphologic variations of foraminifera are regulated by climatic changes remains a mystery. The answer to such changes probably lies in the intricacies of the molecular properties of the protoplasm. However, molecular systematic analyses of foraminifera are yet to start in India. Here, beginning has been made in this direction, by establishing foraminiferal culture program. For that, live specimens (without staining) of benthic foraminiferal species Pararotalia nipponica were collected from the waters off Goa, India. Based on coiling direction and proloculus size, these live specimens were then sorted into four different groups viz., dextral - megalospheric, dextral - microspheric, sinistral - megalospheric and sinistral - microspheric. The specimens of these four groups were then subjected to molecular systematic analysis utilizing 12 S mitochondrial gene, using Polymerase China Reaction (PCR) technique. After the amplification of 12 S gene it was resolved on 1.2% agarose gel containing ethidium bromide. To make the results clear, reamplification was carried out by taking the amplified DNA of the first set. The result shows more or less identifical 12 S mitochondrial gene amplicon for each group. This result does show that as far as 12 S mitochondrial gene is concerned, all the morphologically different specimens, at the level of present experiment may be clubbed together as one species but have limitation in assigning any reason for the morphological differences
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    http://drs.nio.org/drs/handle/2264/387
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