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dc.contributor.authorAparna, A.
dc.contributor.authorArshad, E.
dc.contributor.authorJasmin, C.
dc.contributor.authorPai, S.S.
dc.contributor.authorBrightSingh, I.S.
dc.contributor.authorMohandas, A.
dc.contributor.authorAnas, A.
dc.date.accessioned2012-01-17T09:42:36Z
dc.date.available2012-01-17T09:42:36Z
dc.date.issued2012
dc.identifier.citationMicrobial Biotechnology , vol.5(1); 2012; 59-68
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/4003
dc.description.abstractAntimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems is proposed. The growth of a multiple antibiotic resistant Vibrio harveyi strain was effectively controlled by treating the cells with Rose Bengal and photosensitizing for 30 min using a halogen lamp. This resulted in the death of more than 50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could be attributed to the destruction of outer membrane protein of V. harveyi by reactive oxygen intermediates produced during the photosensitization. Further, mesocosm experiments with V. harveyi and Artemia nauplii demonstrated that in 30 min, the aPDT could kill 78.9% and 91.2% of heterotrophic bacterial and Vibrio population respectively. In conclusion, the study demonstrated that aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large.
dc.language.isoen
dc.publisherSociety for Applied Microbiology
dc.rights© 2011 The Authors Microbial Biotechnology © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
dc.subjectshrimp culture
dc.subjectbacterial diseases
dc.subjectproduct development
dc.subjectviral diseases
dc.titleReducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
dc.typeJournal Article


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