Shivajiella indica gen. nov., sp. nov., a marine bacterium of the family "Cyclobacteriaceae" with nitrate reducing activity
Date
2012Author
AnilKumar, P.
Aravind, R.
Francis, K.
Bhumika, V.
Ritika, C.
Priyashanth, P.
Srinivas, T.N.R.
Metadata
Show full item recordAbstract
Novel orange pigmented, Gram-negative-staining, rod-shaped, non-motile, strictly aerobic strains designated NIO-S1 sup(T) and NIO-S2 were isolated from the water sample of a pond adjacent to the coast and an algal mat from a fish pond, respectively, at Kakinada, India. Both strains were positive for oxidase, catalase and beta-galactosidase activities. The predominant fatty acids in NIO-S1 sup(T) were iso-C sub(15:0) (39.6 percent), anteiso-C sub(15:0) (9.9 percent), iso-C sub(17:0) 3OH (10.9 percent) and C sub(16:1) omega 7c/C sub(16:1) omega 6c (summed feature 3) (5.7 percent). The strains contained MK-7 as the major respiratory quinine, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified lipids as the polar lipids. Phylogenetic analysis indicated that strain NIO-S1 sup(T) was a member of the family "Cyclobacteriaceae" of the class "Sphingobacteriia" and it clustered with the genera Fontibacter, Cecembia and Aquiflexum with phylogenetic distances of 6.8, 9.0 and 12.2 percent (93.2, 91.0 and 87.8 percent similarity), respectively. DNA–DNA hybridization between strains NIO-S1T and NIO-S2 showed a relatedness of 93 percent and rep-PCR banding patterns were similar. Based on data from the current polyphasic study, it is proposed that the new isolates be placed in a new genus and species with the name Shivajiella indica gen. nov., sp. nov. The type strain of Shivajiella indica is NIO-S1 sup(T) (= KCTC 19812 sup(T) = MTCC 11065 sup(T))
Collections
- Scholarly literature [9074]