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    Vibrio harveyi modulated gene expression in Penaeus monodon and Fenneropenaeus indicus

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    Nayak_PhD_thesis.pdf (1.962Mb)
    Date
    2011
    Author
    Nayak, S.
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    Abstract
    This work tries to contribute to a better understanding of the defense system in the post-larvae of P. monodon and F. indicus upon challenge with a virulent strain of V. harveyi. This study involved the use of SSH for construction of subtracted cDNA libraries in order to identify individual genes expressed in the post-larvae as a result of the challenge. Through qPCR analysis, gene expression profiles of different immune related genes in the post-larvae of P. monodon and F. indicus were studied. Furthermore, this study also included full length cloning and characterization of two important immune transcripts: ferritin and translationally controlled tumor protein (TCTP) from the Indian shrimp F. indicus.
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    http://drs.nio.org/drs/handle/2264/4155
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