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dc.contributor.authorFeng, C.
dc.contributor.authorNita-Lazar, M.
dc.contributor.authorGonzalez-Montalban, N.
dc.contributor.authorWang, J.
dc.contributor.authorMancini, J.
dc.contributor.authorRavindran, C.
dc.contributor.authorAhmed, H.
dc.contributor.authorVasta, G.R.
dc.date.accessioned2015-02-11T04:22:39Z
dc.date.available2015-02-11T04:22:39Z
dc.date.issued2015
dc.identifier.citationGalectins:Methods and protocols. ed. by: Stowell, S.R.; Cummings, R.D.. Springer; New York; USA; 2015; 327-341
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/4679
dc.description.abstractTechniques for disrupting gene expression are invaluable tools for the analysis of the biological role(s) of a gene product. Because of its genetic tractability and multiple advantages over conventional mammalian models, the zebrafish (Danio rerio) is recognized as a powerful system for gaining new insight into diverse aspects of human health and disease. Among the multiple mammalian gene families for which the zebrafish has shown promise as an invaluable model for functional studies, the galectins have attracted great interest due to their participation in early development, regulation of immune homeostasis, and recognition of microbial pathogens. Galectins are Beta-galactosyl-binding lectins with a characteristic sequence motif in their carbohydrate recognition domains (CRDs), which comprise an evolutionary conserved family ubiquitous in eukaryotic taxa. Galectins are emerging as key players in the modulation of many important pathological processes, which include acute and chronic inflammatory diseases, autoimmunity and cancer, thus making them potential molecular targets for innovative drug discovery. Here, we provide a review of the current methods available for the manipulation of gene expression in the zebrafish, with a focus on gene knockdown [morpholino (MO)-derived antisense oligonucleotides] and knockout (CRISPR-Cas) technologies.
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofseriesMethods in molecular biology: Methods and protocols; 1207; Walker, J.M.; ed.
dc.rightsCopyright [2015] Springer
dc.subjectgene expression
dc.subjectpublic health
dc.subjectdiseases
dc.subjectDanio rerio
dc.titleManipulating galectin expression in zebrafish (Danio rerio)
dc.typeBook Chapter


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