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dc.contributor.authorNaik, D.
dc.contributor.authorTilvi, S.
dc.contributor.authorDeSouza, L.
dc.date.accessioned2015-07-14T08:35:36Z
dc.date.available2015-07-14T08:35:36Z
dc.date.issued2015
dc.identifier.citationProceedings of 5th Annual International Conference on Advances in Biotechnology (BIOTECH 2015), 13-15th March 2015. ed. by: Kumar, A.; IIT Kanpur; India; 2015; 180-186
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/4744
dc.description.abstractPseudomonas aeruginosa is a major nosocomial pathogen which causes hospital acquired infections and recently has gained importance as a model to study antibiotic resistance. In the present study, we investigated the effect of methanol and methanol: chloroform (1:1) extracts of 7 mangrove plants on P. aeruginosa ATCC 27853 motilities (swarming, swimming and twitching). Amongst the 22 extracts tested, methanolic extract of Rhizophora conjugata fruit showed maximum inhibition. The butanol (RcBu) fraction of this extract almost completely inhibited swarming and swimming motility by 86% each and twitching by 90%, at a concentration of 100mug mL<sup>-1</sup>. This fraction also showed maximum reduction in the production of pyocyanin with 57% inhibition followed by aqueous (RcAq) fraction with 46%. The wetness of the P. aeruginosa colony was reduced in the presence of RcBu and RcAq fractions with percentage inhibition of 64% and 60%, respectively. However, none of the fractions showed significant reduction in rhamnolipid production. The metabolites present in the most active fraction were identified as polymeric flavan-3-ol of catechin/epicatechin and glucoside flavonoid, rutin (2-6) using electrospray ionizationtandem mass spectrometry (ESI-MS/MS). The isolation of (+)- epiafzelechin (1) from of ethyl acetate (RcEA) fraction is also reported here for the first time.
dc.language.isoen
dc.publisherGSTF
dc.rightsCopyright [2015]. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository.
dc.subjectMICROBIOLOGY::General
dc.subjectAQUATIC RESOURCES::Natural products
dc.titleFlavonoids from Rhizophora conjugata fruit extract blocks virulence factors of Pseudomonas aeruginosa
dc.typeConference Article


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