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    Exploring rhizosphere bacteria of Eichhornia crassipes for metal tolerance and biological activity

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    Biotech_2015_1.pdf (5.449Mb)
    Date
    2015
    Author
    PrabhaDevi
    Gomez, S.
    Ribeiro, M.
    Deshpande, S.A.
    Singh, K.S.
    DeSouza, L.
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    Abstract
    With urbanization and rapid industrial progress, water bodies around the world are getting highly polluted with metals. Bacteria have evolved several mechanisms to adapt to heally metal contaminants thus representing a potential source for bioremediation process. The present study deals with isohtion, identifiation and characterization of metal tolerant bacteria(MTB) from the rhizosphere of Eichhornia crassipes (water hyacinth) collected from the backwaters of Cochin (South India, 9°58'N, 76°l7'E). Depending on the metals they tolreted (Pb, Cu, Fe, Zn. & Co), the metal tolerant bacteria were labeled as Pb-1 to Pb-3, Cu-1 to Cu-4, Fe-1 to Fe-4,Zn-1 to Zn-4, and Co-1 to Co-2. No cultures tolerated and grew in the presence of Hg and Cd. Cultures were identified on the basis of 16s rRNA gene sequence analysis. Morphological and compositional studies were conducted using computer-assisted Scanning Electron Microscopy equipped with Energy Dispersive Spectroscopy (SEM/EDS). The maximum tolerable concentration (MTC) which is the highest concentration having minimum effect on the growth of the isolates were determined and found to be <2mM for CuSO<sub>4</sub>, <3mM for ZnSO<sub>4</sub> <3.5mM for CoCl<sub>2</sub>, <12mM for PbNO<sub>3</sub> and 25mM for FeCl<sub>3</sub>, They were further screened for antibiotic sensitivity and biological activity according to Kirby-Bauer disc diffusion method The MTB under metal stress condition showed significant biological activity against clinical pathogens, fouling bacteria and selective plant and fish pathogens indicating their biotechnological application in medicine, agriculture and aquaculture.
    URI
    http://drs.nio.org/drs/handle/2264/4751
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