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dc.contributor.authorGaonkar, C.C.
dc.contributor.authorKhandeparker, L.
dc.contributor.authorDesai, D.V.
dc.contributor.authorAnil, A.C.
dc.date.accessioned2015-08-24T09:41:10Z
dc.date.available2015-08-24T09:41:10Z
dc.date.issued2015
dc.identifier.citationJournal of the Marine Biological Association of the United Kingdom, vol.95(3); 2015; 497-502
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/4756
dc.description.abstractIdentification of marine invertebrate larvae using morphological characters is laborious and complicated by phenotypic plasticity. Balanus amphitrite is a dominant barnacle, important in the context of intertidal ecology and biofouling of manmade structures. Morphological identification of barnacle larval forms in a mixed population is difficult because of their intricacy and similarity in size, shape and developmental stages. We report the development and application of a nucleic acid-based Polymerase Chain Reaction (PCR) method for the specific identification of the barnacle, B. amphitrite, from the heterogeneous zooplankton sample. This method is reliable and accurate thereby overcoming taxonomic ambiguity. Sequence alignment of the 18S rRNA gene region of selected species of barnacles allowed the design of B. amphitrite-specific PCR primers. Assay specificity was evaluated by screening DNA obtained from selected species of barnacles. The oligonucleotide primers used in the study flanked a 1600 bp region within the 18S rRNA gene. The primer is specific and can detect as few as 10 individuals of B. amphitrite larvae spiked in a background of ~186 mg of zooplankton. This technique facilitates accurate identification and the primer can be used as a marker for enumeration of B. amphitrite larvae in the plankton.
dc.language.isoen
dc.publisherMarine Biological Association of the United Kingdom
dc.rightsAn edited version of this paper was published © Marine Biological Association of the United Kingdom, 2015. All efforts have been made to respect the copyright to the best of our knowledge. Inadvertent omissions, if brought to our notice, stand for correction and withdrawal of document from this repository
dc.subjectAQUATIC COMMUNITIES::Zooplankton
dc.subjectINVERTEBRATE BIOLOGY::Taxonomy and morphology
dc.subjectCRUSTACEANS::Taxonomy and morphology
dc.titleIdentification of Balanus amphitrite larvae from field zooplankton using species-specific primers
dc.typeJournal Article


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