Genotoxic potency of mercuric chloride in gill cells of marine gastropod Planaxis sulcatus using comet assay

Abstract

In vivo and in vitro exposures were used to investigate the genotoxicity of mercuric chloride (HgCl₂) to the marine snail, Planaxis sulcatus. The comet assay protocol was validated on gill cells exposed in vitro to hydrogen peroxide (H₂O₂, 0–50 μM). Snails were exposed in vivo for 96 h to HgCl₂ (10, 20, 50, and 100 μg/l). Our results showed significant concentration-dependent increase in the tail DNA (TDNA) and olive tail moment (OTM) in exposed snails for all doses compared with controls. In vitro exposure to HgCl₂(10–100 μg/l) resulted in significantly higher values for TDNA at all concentrations. Our results showed that DNA damage increased in the gill cell with increasing exposure time. This study demonstrates the usefulness of comet assay for detection of DNA damage after exposure to HgCl₂ and the sensitivity of marine snail P. sulcatus as a good candidate species for metal pollution