Marinobacter nitratireducens sp. nov., a halophilic and lipolytic bacterium isolated from coastal surface sea water
Date
2015Author
Bhumika, V.
Ravinder, K.
Korpole, S.
Srinivas, T.N.R.
AnilKumar, P.
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A novel Gram-stain-negative, rod-shaped, motile bacterium, designated strain AK21<sup>T</sup> , was isolated from coastal surface sea water at Visakhapatnam, India. The strain was positive for oxidase, catalase, lipase, L-proline arylamidase and tyrosine arylamidase activities. The predominant fatty acids were C<sub>12:0</sub>, C<sub>12:0</sub> 3-OH, C<sub>16:0</sub>, C<sub>16:1</sub>w9c, C<sub>18:1</sub>v9c and summed feature 3 (C16:1v7c and/or iso-C<sub>15:0</sub> 2-OH). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, two unidentified phospholipids and one unidentified lipid. Q-10 was the predominant respiratory quinone. The DNA G+C content of the strain was 54.6 mol%. 16S rRNA gene sequence analysis indicated that strain AK21<sup>T</sup> was a member of the genus Marinobacter and was closely related to Marinobacter xestospongiae, with pairwise sequence similarity of 97.2 % to the type strain, with similarity to other members of the genus of 94.0–96.8 %. The mean DNA–DNA relatedness of strain AK21<sup>T</sup> with M. xestospongiae JCM 17469T was 34.5 %, and relatedness with Marinobacter mobilis JCM 15154<sup>T</sup> was 40.5 %. Phylogenetic analysis showed that strain AK21<sup>T</sup> clustered with the type strains of M. xestospongiae and M. mobilis at distances of 2.9 and 2.8 % (97.1 and 97.2 % similarity), respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain AK21<sup>T</sup> represents a novel species of the genus Marinobacter, for which the name Marinobacter nitratireducens sp. nov. is proposed. The type strain of Marinobacter nitratireducens is AK21<sup>T</sup> (5MTCC 11704<sup>T</sup> 5JCM 18428<sup>T</sup> )
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