Comparative in vitro study on free radical scavenging potential of selected bivalve species
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Bivalves such as, Crassostrea spp., Meretrix casta, Placuna placenta and Polymesoda erosa are largely consumed as edible seafood. It forms natural source of nutrition in coastal and worldwide population. Free radical scavenging activities of these species were tested against 2, 2-diphenyl-1-picryl-hydrazyl (DPPH), hydroxyl radical (OH), reducing power and in vitro inhibition of lipid peroxidation (LPX). Methanolic extracts exhibited dose dependency with increasing concentration (3 to 9 mg/ml) of sample. On comparing DPPH activities, M. casta and P. placenta statistically exhibited significant radical scavenging properties (p less than 0.01). However, scavenging effect of Crassostrea spp. was higher irrespective of dose dependency. Stable hydroxyl scavenging effects and increasing reducing actions with increase in absorbance were also seen to be concentration dependant. Superior activities against in vitro induced LPX were observed in the order of P. placenta more than M. casta more than P. erosa more than Crassostrea spp. This indicates protective role against deterioration cell membrane lipids during oxidative stress. Moreover, correlation of variables (p less than 0.001) in the present study, further supports our views on, M. casta and Crassostrea spp. as natural source of antioxidative seafood.
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