dc.contributor.author | Vrinda, S. | |
dc.contributor.author | Jasmin, C. | |
dc.contributor.author | Sivakumar, K.C. | |
dc.contributor.author | Jose, S. | |
dc.contributor.author | Jose, B. | |
dc.contributor.author | Philip, R. | |
dc.contributor.author | BrightSingh, I.S. | |
dc.date.accessioned | 2017-03-01T08:53:19Z | |
dc.date.available | 2017-03-01T08:53:19Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Comparative Biochemistry and Physiology, Part A, vol.203; 2017; 167-178 | |
dc.identifier.uri | http://drs.nio.org/drs/handle/2264/5076 | |
dc.description.abstract | Gonad inhibiting hormone (GIH), type II class of the CHH family neuropeptides, is released by the neurohaemal XO-SG complex of the eyestalk. The inhibitory function of GIH has a pivotal role in gonad development and reproduction. In this study, we report the expression and production of a thioredoxin-fused mature GIH protein (mf-PmGIH) of Penaeus monodon in a bacterial system and its use as antigen to raise polyclonal antiserum (anti-mf-PmGIH). The mature GIH gene of 237 bp that codes for 79 amino acids, was cloned into the Escherichia coli thioredoxin gene fusion expression system. The expression vector construct (mf-PmGIH + pEt32a +) upon induction produced 32.16 kDa mature GIH fusion protein (mf-PmGIH)·The purified fusion protein was used as exogenous GIH and as antigen to raise polyclonal antisera. The fusion protein when injected into juvenile shrimp significantly reduced vitellogenin/vitellin levels by 31.55% within 72 h in comparison to the controls showing the gonad inhibiting property. Vitellogenin/vitellin levels were significantly induced by 74.10% within 6 h when polyclonal antiserum (anti-mf-PmGIH-1:500) was injected in P.monodon. Anti-mf-PmGIH immunolocalized GIH producing neurosecretory cells in the eyestalk of P.monodon. The present manuscript reports an innovative means of gonad inhibition and vitellogenin/vitellin induction with thioredoxin fused GIH and antisera developed | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.rights | An edited version of this paper was published by Elsevier. Copyright [2016] Elsevier | |
dc.subject | Crustaceans::Physiology, biochemistry, biophysics | |
dc.subject | Crustaceans::Reproduction and development | |
dc.title | Regulating gonad inhibition and vitellogenin/vitellin induction in Penaeus monodon using mature GIH fusion protein and polyclonal antisera | |
dc.type | Journal Article | |