Kocuria indica sp nov., isolated from sediment sample
Date
2014Author
Dastager, S.G.
Tang, S.K.
Krishnamurthi, S.
Lee, J.C.
Li, W.-J.
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A actinobacterial strain isolated from a sediment sample from Chorao Island, in Goa province India, and subjected to a taxonomic investigation. The isolate, designated NIO-1021T, was a Gram-positive, aerobic, non-motile and coccoid. Strain NIO-1021T was identified as a member of the genus Kocuria by a polyphasic approach. Strain NIO-1021T could be differentiated from other members of the genus Kocuria on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain NIO-1021T was being most closely related to Kocuria marina (98.6%) similarity with 19 nucleotide differences respectively. Further DNA-DNA hybridization analysis revealed that they have lower relatedness with nearest type strains. The strain formed a monophyletic clade with K. marina with 100% bootstrap values. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified lipids. The predominant menaquinone was MK-7(H2). The major fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0. The DNA G+C content of strain NIO-1021T was 60.5 mol%. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification in the genus Kocuria. Based on phenotypic, chemotypic and molecular characteristics, strain NIO-1021T represents a novel species of the genus Kocuria, for which the name Kocuria indica sp. nov. is proposed, with type strain NIO-1021T(=NCIM 5455T= DSM 25126T =CCTCC AA 209050T) as the type strain
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