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dc.contributor.authorKumaran, S.P.
dc.contributor.authorKutty, B.C.
dc.contributor.authorChatterji, A.
dc.contributor.authorParameswaran, P.S.
dc.contributor.authorMishra, K.P.
dc.date.accessioned2008-08-07T10:20:41Z
dc.date.accessioned2017-09-30T03:07:59Z
dc.date.available2008-08-07T10:20:41Z
dc.date.available2017-09-30T03:07:59Z
dc.date.issued2007
dc.identifier.citationJournal of Environmental Pathology Toxicology and Oncology, vol.26(4); 2007; 263-273
dc.identifier.issn0731-8898
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/7583
dc.description.abstractThis study describes the radioprotective ability of a hydrolysate prepared using an enzymeacid hydrolysis method from the green mussel Perna viridis in terms of its ability to prevent radiation-induced damage in plasmid DNA, cell death, reactive oxygen species (ROS) formation, and DNA damage in mice lymphocytes The mussel hydrolysate (MH) present during irradiation showed significant protection from Gamma-radiation-induced strand breaks in plasmid DNA as evaluated by gel electrophoresis Viability studies by trypan blue dye exclusion and MTT assay showed that pre incubation of mice splenic lymphocytes with MH protected them from Gamma-radiation mediated killing Moreover, the presence of MH during irradiation of isolated mice lymphocytes significantly decreased the DNA damage, as measured by comet assay Measurement of intracellular ROS by dichlorofluorescein fluorescence revealed that the presence of MH effectively reduced the ROS generated in lymphocytes by both chemical method and Gamma-irradiation Prevention of DNA damage both in plasmid and lymphocytes and cell death in lymphocytes appears correlated with reduction of oxidatively generated free radicals It is concluded that protection against radiation-induced cell death and DNA damage by MH was attributable to reduction of reactive free radical species generated by Gamma-radiation
dc.languageen
dc.publisherBegell House
dc.relation.ispartofJ Environ Pathol Tox
dc.relation.isreferencedbySCI
dc.subjectGeology and Geophysics
dc.subject.othermarine molluscs
dc.subject.otherDNA
dc.subject.otherplasmids
dc.subject.otherbiological damage
dc.subject.otherchemical extraction
dc.subject.otherPerna viridis
dc.subject.otherISW, India, Goa
dc.titleRadioprotection against DNA damage by an extract of Indian green mussel, Perna viridis (L.)
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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