Oceanospirillum sanctuarii sp. nov., isolated from a sediment sample
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A novel Gram-staining-negative, spiral-shaped, pale-yellow, non-sporulating, motile, aerobic bacterium, designated strain AK56T, was isolated from a sediment sample collected at the Coringa Wildlife Sanctuary, India. Colonies on marine agar were circular, pale yellow, shiny, translucent, 1-2?mm in diameter, convex and had an entire margin. The major fatty acids included C16?:?1, C16?:?1 ?7 c/C16?:?1?6c and C18?:?1?7c. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and five unidentified lipids. DNA-DNA hybridization between strain AK56T and� Oceanospirillum linum LMG 5214T and � Oceanospirillum nioense � NIO-S6 showed relatedness values of 39.91 and 23.62?%,?respectively. The DNA G+C content of strain AK56T was found to be 50.3?mol%. A sequence similarity search for the 16S rRNA gene sequence revealed that� O. linum and� O. nioense were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 98.9 and 98.2?%,?respectively. Phylogenetic analysis also showed the formation of a cluster including strain AK56T with close relative� O. linum and� O. nioense . Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK56T is described in this study as a novel species in the genus� Oceanospirillum , for which the name Oceanospirillum sanctuarii sp. nov. is proposed. The type strain of� Oceanospirillum sanctuarii is AK56T (=MTCC 12005T=JCM 19193T=KCTC 52973T).
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