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dc.contributor.authorGajbhiye, D.S.
dc.contributor.authorKhandeparker, L.
dc.date.accessioned2018-06-06
dc.date.accessioned2018-10-23T09:24:56Z
dc.date.available2017-08-01
dc.date.available2018-10-23T09:24:56Z
dc.date.issued2017
dc.identifier.citationMarine Environmental Research, vol.129; 2017; 14-23
dc.identifier.issn0141-1136
dc.identifier.urihttps://doi.org/10.1016/J.MARENVRES.2017.04.009
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/7822
dc.description.abstractPaphia malabarica is a predominant and commercially important bivalve in India, persistently challenged by wavering salinity in a monsoon-influenced estuary. To examine the organism's immunological response under such a condition we challenged P. malabarica with different salinities (0, 5, 15, 25 and 35) for varied periods using a two-way experimental approach (in vitro and in vivo). This is the first study to report the response of P. malabarica hemocytes to salinity stress from a monsoon-influenced estuary on the southwest coast of India. Evaluation of total hemocytes count, mortality, lysosomal content, reactive oxygen species production, phagocytic and esterase activity was carried out using flow cytometric analysis. In both the experimental conditions, hemocyte parameters were significantly compromised at lower salinities (0 and 5) with an evident immuno-salinity tolerance range of 15-35. The damaging impact of 0 and 5 salinities on hemocyte function intensified with a longer exposure period, indicating that prolonged exposure to low salinity could be detrimental to bivalve wellness if they are pushed beyond their tolerance range which is usually observed during the monsoon. Further studies should focus on the interactive effect of salinity tagged with different stressors influencing biology of P. malabarica.
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMar Environ Res
dc.relation.isreferencedbySCI
dc.titleImmune response of the short neck clam Paphia malabarica to salinity stress using flow cytometry
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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