Bacillus mangrovi sp. nov., isolated from a sediment sample from a mangrove forest
Date
2017Author
Gupta, Vasundhera
Singh, P.K.
Korpole, S.
Srinivas, T.N.R.
AnilKumar, P.
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A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5?mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48?h growth at 30?�C. Growth occurred at 15-42?�C, with 0-3?% (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities. The fatty acids were dominated by branched types with iso- and anteiso- saturated fatty acids with a high abundance of iso-C14?:?0, iso-C15?:?0, anteiso-C15?:?0 and iso-C16?:?0; the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid; and MK-7 was the major menaquinone. DNA-DNA hybridization between AK61T and� Bacillus indicus MTCC 4374T and between AK61T and� Bacillus indicus KCTC 3880 showed relatedness of 37.99 and 33.32?%?respectively. The DNA G+C content of AK61T was 44?mol%. The results of a blast sequence similarity search based on 16S rRNA gene sequences indicated that� Bacillus cibi and� Bacillus indicus were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 97.69 and 97.55?%?respectively. The results of phylogenetic analysis indicated that AK61T was clustered with� Bacillus idriensis and� Bacillus indicus . On the basis of its phenotypic characteristics and phylogenetic inference, AK61T represents a novel species of the genus� Bacillus , for which the name Bacillus mangrovi sp. nov. is proposed. The type strain is AK61T (=JCM 31087T=MTCC 12015T=KCTC 33872T).
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