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dc.contributor.authorKhandeparker, R.
dc.contributor.authorParab, P.
dc.contributor.authorAmberkar, U.
dc.date.accessioned2018-06-06
dc.date.accessioned2018-10-23T09:24:58Z
dc.date.available2017-06-14
dc.date.available2018-10-23T09:24:58Z
dc.date.issued2017
dc.identifier.citationFood Technology and Biotechnology, vol.55(2); 2017; 164-172
dc.identifier.issn1334-2606
dc.identifier.urihttps://doi.org/10.17113/ftb.55.02.17.4896
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/7844
dc.description.abstractBacterial strain Bacillus tequilensis BT21 isolated from marine sediments was found to produce extracellular xylanase. The xynBT21 gene encoding xylanase enzyme was cloned and expressed in Escherichia coli. The gene encoded a protein consisting of 213 amino acid residues with calculated molecular mass of 23.3 kDa. Puri?ed recombinant xylanase had optimum activity at 60 °C and pH=6. The enzyme was highly stable in alkaline pH, at pH=7 it remained 100 % active for 24 h, while its activity increased at pH=8 and 9 during incubation. B. tequilensis BT21 xylanase had alkaline pI of 9.4 and belongs to glycosyl hydrolase family 11. The mode of action of XynBT21 on beechwood xylan and xylooligosaccharides was studied. It hydrolysed xylooligosaccharides and beechwood xylan yielding mainly xylobiose (X2) with a small amount of xylose (X1), indicating that XynBT21 was probably an endo-acting xylanase. Enzymatic hydrolysis using wheat bran as a substrate revealed that xylanase reported here has the potential to produce xylobiose from wheat bran. Xylooligosaccharides, especially xylobiose, have strong bifidogenic properties and are increasingly used as a prebiotic. This is the first report that describes this novel xylanase enzyme from marine B. tequilensis BT21 used for the release of xylobiose from wheat bran.
dc.language.isoen
dc.publisherUniversity of Zagreb, Croatia
dc.relation.ispartofFood Technol Biotechnol
dc.relation.isreferencedbySCI
dc.titleRecombinant Xylanase from Bacillus tequilensis BT21: Biochemical Characterization and Its Application in Production of Xylobiose from Agricultural Residues
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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