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dc.contributor.authorTilvi, S.
dc.contributor.authorPrabhaDevi
dc.contributor.authorMajik, M.S.
dc.date.accessioned2018-06-06
dc.date.accessioned2018-10-23T09:24:58Z
dc.date.available2017-06-07
dc.date.available2018-10-23T09:24:58Z
dc.date.issued2017
dc.identifier.citationEuropean Journal of Mass Spectrometry, vol.23(3); 2017; 92-97
dc.identifier.issn1751-6838
dc.identifier.urihttps://doi.org/10.1177/1469066717699218
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/7847
dc.description.abstractButanol fraction of sacoglossan Elysia grandifolia was investigated for identifying peptides using electrospray ionisation-tandem mass spectrometry (ESI-MS/MS). Without prior isolation, the structural determination is achieved on the basis of mass fragmentation pattern and comparison with the previously established data. The ESI-MS of the fraction in the positive ion mode gave clusters of singly and doubly charged molecular ion peaks. The ESI-MS spectrum showed peaks for the presence of the peptides kahalalides F, G, R and S reported earlier. In addition, it also showed molecular ion peaks at m/z 1557.8 [M+H]+ and doubly charged ions at m/z 779.4 [M+2H]2+, 790.4 [M+Na]2+ and 796.4 [M+K]2+. The MS/MS at m/z 779.4 [M+2H]+2 at collision energy 40?V obtained series of b and y fragment ions. The MS/MS spectrum showed identical fragment ion y6 at m/z 643 which revealed that cyclic part is identical with kahalalide F, R and S. Careful examination of the fragment ions b1 to b7 with their corresponding y fragment ions y12 to y6, respectively and by comparison of MS/MS pattern of kahalalide S, established that proline can be replaced by tyrosine amino acid residue. The mass difference between b4 (m/z 511) and b5 (m/z 674) is equal to 163 which is equivalent to mass residue of tyrosine. Their y fragment ions also quickly helped in fixing the puzzle. This resulted in the identification of the peptide sequence cyclo-[Val-(5-MeHex-Val-Thr-Val-Val-Tyr-Lys-Ile)Thr-Ile-Val-Phe-Dhb)] for the new cyclodepsipeptide, kahalalide Z3. Thus, ESI-MS/MS has set a trend in quick identification of new marine molecules.
dc.language.isoen
dc.publisherSage Publications
dc.relation.ispartofEur J Mass Spectrom
dc.relation.isreferencedbySCI
dc.titleQuick elucidation of cyclodepsipeptide sequence from sacoglossan Elysia grandifolia using electrospray ionisation-tandem mass spectrometry
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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