• Login
    View Item 
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Quantitative analyses of denitrifying bacterial diversity from a seasonally hypoxic monsoon governed tropical coastal region

    Thumbnail
    Date
    2018
    Author
    Gomes, J.
    Khandeparker, R.
    Bandekar, M.
    Meena, R.M.
    Ramaiah, N.
    Metadata
    Show full item record
    Abstract
    Microbial community mediated denitrification leads to nitrate reduction and, its eventual removal from marine ecosystems. Therefore, it is necessary to understand the functional diversity in terms of the abundance and types of nitrate reductases in the microbial communities extant in the hypoxic/suboxic waters where organic matter oxidation is predominantly nitrate- reduction mediated. For this study, water samples from the surface, mid-depth and close to bottom were collected from three coastal locations along the west coast of India during Summer monsoon (SuM), Fall intermonsoon (FIM) and, Spring intermonsoon (SIM). The abundance of nitrate (narG), nitrite (nirS) and nitrous oxide (nosZ) reductase genes from these water samples were quantified by qPCR method. Besides the distinct spatio-temporal differences, the overall ranges of these three functional genes across the sampling sites ranged from 1.9 x 107 to 7.9 x 107 copies L-1 (narG), from 0.001 x 106 to 0.30 x 106 copies L-1 (nirS) and from 0.30 x 106 to 2.9 x 106 copies L-1 (nosZ). Functional gene sequencing of bacterial communities possessing these reductases were diverse and, dominated by members of Alpha-, Beta- and Gamma-proteobacteria, including taxonomic groups containing well known denitrifiers such as Pseudogulbenkiania, Kocuria, Pseudomonas, Herbaspirillum, Achromobacter, Rhodoferax, Mesorhizobium, Sinorhizobium, Shinella, Bradyrhizobiaceae, Rhodopseudomonas, Dinoroseobacter, Paracoccus, Rhodobacter, Ruegeria and Azospirillum sp. Assemblages of bacteria possessing narG, nirS, and nosZ indicated that complete denitrification could occur, particularly in the samples collected close to the bottom.
    URI
    https://doi.org/10.1016/J.DSR2.2017.12.012
    http://drs.nio.org/drs/handle/2264/7983
    Collections
    • Scholarly literature [9074]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV