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    Bacillus alkalilacus sp. nov., isolated from a sediment sample from a lake in India

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    Intern_Jou_System_Evolut_Microb_68_1665a.pdf (1013.Kb)
    Date
    2018
    Author
    Singh, H.
    Kaur, M.
    Sharma, S.
    Kaur, L.
    Mishra, Sunita
    Srinivas, T.N.R.
    AnilKumar, P.
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    Abstract
    An aerobic, endospore-forming, haloalkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK73T, was isolated from a sediment sample collected from Sambhar lake, Jaipur, Rajasthan, India. Colonies were circular, 1-2-mm in diameter, glossy, smooth, yellowish and convex with an entire margin after 48-h growth on marine agar at pH 9 and 37oC. Growth occurred at 15-42oC, 0-10-% (w/v) NaCl and at a pH range of 7-12. Strain AK73T was positive for catalase and arginine dihydrolase 2 activities, hydrolysis of Tweens 20, 40 and 80, and negative for esculinase, caseinase, gelatinase, --galactosidase, lipase (Tween 60) and urease activities. The fatty acids were dominated by branched iso-, anteiso-, saturated fatty acids with a high abundance of iso-C15-:-0, anteiso-C15-:-0, C16-:-0 and anteiso-C17-:-0; MK-7 was the major menaquinone. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, four unidentified phospholipids and three unidentified lipids. The DNA G+C-content of strain AK73T was 54-mol%. Analysis based on comparative 16S rRNA gene sequence analysis indicated that Bacillus alcalophilus was the nearest phylogenetic neighbour, with a pair-wise sequence similarity of 96.0-%. Phylogenetic analysis showed that strain AK73T formed a separate lineage but was loosely associated with a peripheral cluster of organisms that contained Bacillus gibsonii , Bacillus murimartini and Bacillus plakortidis with similarity values of 93.6, 93.5 and 93.4-%, respectively. Based on its phenotypic characteristics and on phylogenetic inference, strain AK73T represents a novel species of the genus Bacillus , for which the name Bacillus alkalilacus sp. nov. is proposed. The type strain is AK73T (=JCM 32184T=MTCC 12637T=KCTC 33880T)
    URI
    https://doi.org/10.1099/ijsem.0.002726
    http://drs.nio.org/drs/handle/2264/8071
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