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dc.contributor.authorGajbhiye, D.S.
dc.contributor.authorKhandeparker, L.
dc.date.accessioned2019/04/29
dc.date.accessioned2019-04-30T12:48:24Z
dc.date.available2019/01/01
dc.date.available2019-04-30T12:48:24Z
dc.date.issued2019
dc.identifier.citationFish & Shellfish Immunology, vol.84; 2019; 384-389
dc.identifier.issn1050-4648
dc.identifier.urihttps://doi.org/10.1016/J.FSI.2018.10.025
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/8110
dc.description.abstractIn recent years, the role of inducible nitric oxide synthase (iNOS) isoform has been widely studied because of its immunological relevance in higher organisms as well as invertebrates including bivalves. However, little is known about the immunological role of iNOS in Paphia malabarica defense mechanism. In this study, we immunodetected the presence of iNOS in P. malabarica hemocytes using antibody N9657 monoclonal anti-nitric oxide synthase. In addition, increased iNOS activity was evident in response to a higher bacterial dosage (Vibrio parahaemolyticus and V. cholerae), highlighting the dose-dependent iNOS activity induction. Also, higher bacterial survivability was observed in the presence of iNOS inhibitor, i.e., S-methylisothiourea hemisulphate (SMIS) thus, validating the bactericidal role of iNOS. These findings implicate the involvement of iNOS in immune-functioning of P. malabarica. Future work should focus on elucidating the expression and regulation of pathogenesis in P. malabarica, involving iNOS.
dc.languageen
dc.publisherElsevier
dc.relation.isreferencedbySCI
dc.titleInvolvement of inducible nitric oxide synthase (iNOS) in immune-functioning of Paphia malabarica (Chemnitz, 1782)
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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