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dc.contributor.authorKrishnaswamy, A.
dc.contributor.authorBarnes, N.
dc.contributor.authorLotlikar, N.P.
dc.contributor.authorDamare, S.R.
dc.date.accessioned2019/04/29
dc.date.accessioned2019-04-30T12:48:24Z
dc.date.available2019/01/26
dc.date.available2019-04-30T12:48:24Z
dc.date.issued2019
dc.identifier.citationIndian Journal of Microbiology, vol.59(1); 2019; 100-104
dc.identifier.issn0973-7715
dc.identifier.urihttps://doi.org/10.1007/s12088-018-0752-y
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/8113
dc.description.abstractFilamentous fungi are ubiquitous eukaryotes having chitin as a major constituent of the cell wall. Chitin is tough to lyse due to which the intracellular fungal proteins are not readily accessible. The problem is further enhanced when the biomass to be analyzed for protein studies is too little due to the extreme experimental parameters under consideration such as increased or lowered pH, temperature, hydrostatic pressure, nutrients, etc. The method described here is capable of obtaining proteins from minuscule quantities of biomass (~5 mg lyophilized biomass). In this study, different lysing conditions and varied composition of extraction buffers were tried to obtain maximum protein of high quality. Lysis with zirconium beads in a combination buffer system (Tris-MgCl2 buffer, urea buffer I and urea buffer II) was best for extracting proteins from the fungal isolates used. The protocol described here provides for a simple and quick method for extraction of high-quality proteins from very less biomass that could be extended to other tough to lyse biological material also
dc.languageen
dc.publisherSpringer India
dc.relation.isreferencedbySCI
dc.titleAn Improved Method for Protein Extraction from Minuscule Quantities of Fungal Biomass
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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