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dc.contributor.authorJaya, P.
dc.contributor.authorNathan, V.K.
dc.contributor.authorParvathi, A.
dc.date.accessioned2019-12-31
dc.date.accessioned2020-02-04T09:38:21Z
dc.date.available2019-10-21
dc.date.available2020-02-04T09:38:21Z
dc.date.issued2019
dc.identifier.citationPreparative Biochemistry and Biotechnology, vol.49(9); 2019; 891-899
dc.identifier.urihttps://doi.org/10.1080/10826068.2019.1633669
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/8230
dc.description.abstractThe novel technology of biological carbon sequestration using microbial enzymes have numerous advantages over conventional sequestration strategies. In the present study, extracellular carbonic anhydrase (CA) producing bacteria were isolated from water samples in the Arabian Sea, India. A potential isolate, Bacillus safensis isolate AS-75 was identified based on 16S rDNA sequence analysis. The culture conditions suitable for CA production were 32 �C incubation temperature with 4% NaCl and 10 mM Zn supplementation. Experimental optimization of culture conditions enhanced enzyme activity to 265 U mL?1. CA specific gene was characterized and based on the analysis, the CA of B. safensis isolate AS-75 was a leucine (11.3%) with ?-helices as the dominant component in its secondary structure. Based on soil microcosm studies, CA could sequester CO2 by 95.4% � 0.11% in sterilized soil with enzyme microcosm. Hence, the application of enzyme was found to be more effective in removing CO2.
dc.languageen
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleCharacterization of marine bacterial carbonic anhydrase and their CO2 sequestration abilities based on a soil microcosm
dc.typeJournal Article


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