• Login
    View Item 
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Extinction of Antimicrobial Resistant Pathogens Using Silver Embedded Silica Nanoparticles and an Efflux Pump Blocker

    Thumbnail
    View/Open
    ACS_App_Bio_Mate_2_4681a.pdf (2.143Mb)
    Date
    2019
    Author
    Jose, J.
    Anas, A.
    Jose, B.
    Puthirath, A.B.
    Athiyanathil, S.
    Jasmin, C.
    Anantharaman, M.R.
    Nair, S.
    Subrahmanyam, C.
    Biju, V.
    Metadata
    Show full item record
    Abstract
    Control measures against antimicrobial resistant bacterial pathogens are important challenges in our daily life. In this study, we discuss the sensitivity and resistance of four bacterial pathogens, Vibrio alginolyticus, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, to silver-silica hybrid nanoparticles. Successively, by combining with an efflux pump blocking agent Verapamil, we find that these hybrid nanoparticles induce complete mortality to even the most resistive S. aureus. The above pathogens are selected from a pool of 100 bacterial strains resistant to silver nitrate. While S. aureus shows increased resistance to the nanoparticles, the cell wall integrity and genetic stability of V. alginolyticus and E. coli are compromised in the presence of the hybrid nanoparticles. These studies suggest that the antimicrobial properties of the nanoparticles against Gram-negative pathogens originate from increased oxidative stress, which is confirmed by the blocking of reactive oxygen species (ROS) using scavengers such as ascorbic acid and observing DNA damage. The antimicrobial property of the nanoparticle when combined with its nontoxic nature to mammalian cells makes it a promising agent for controlling drug-resistant Gram-negative pathogens.
    URI
    https://doi.org/10.1021/acsabm.9b00614
    http://drs.nio.org/drs/handle/2264/8292
    Collections
    • Scholarly literature [9074]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV