dc.contributor.author | Damare, S.R. | |
dc.contributor.author | Mishra, A. | |
dc.contributor.author | DeSouza-Ticlo-Diniz, D. | |
dc.contributor.author | Krishnaswamy, A. | |
dc.contributor.author | Raghukumar, C. | |
dc.date.accessioned | 2020-12-31 | |
dc.date.accessioned | 2021-02-09T10:13:11Z | |
dc.date.available | 2020-11-11 | |
dc.date.available | 2021-02-09T10:13:11Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | 3 Biotech, vol.10(12); 2020; Article no: 528 | |
dc.identifier.issn | 2190-5738 | |
dc.identifier.other | https://link.springer.com/article/10.1007/s13205-020-02520-x | |
dc.identifier.uri | https://doi.org/10.1007/s13205-020-02520-x | |
dc.identifier.uri | http://drs.nio.org/drs/handle/2264/8386 | |
dc.description | IF2019 - 1.798 | |
dc.description.abstract | We report here the production of an alkaline serine protease by Aspergillus flavus isolated at 5600-m depth from deep-sea sediments of the Central Indian Basin. When grown on defatted groundnut oil meal at 30��C for 48-72�h, this fungal isolate produced 2000-2500 ACU mL-1 of alkaline protease. The purified protease had activity optima at pH 10.0 and 45��C. It was a thiol-independent serine protease, identified as an alkaline serine protease ALP1 with a molecular mass of 42.57�kDa. The thermostability and activity of the enzyme increased at 60��C, in the presence of additives such as sucrose, Tween 20, sorbitol, Ca2+ and glycerol and was not adversely affected by H2O2 indicating its potential as a detergent additive. | |
dc.language | en | |
dc.publisher | Springer Science and Business Media Deutschland GmbH | |
dc.relation.ispartof | 3 Biotech | |
dc.relation.isreferencedby | SCI | |
dc.rights | Only the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations | |
dc.title | A deep-sea hydrogen peroxide-stable alkaline serine protease from Aspergillus flavus | |
dc.type | Journal Article | |
dc.affiliation.author | CSIR-National Institute of Oceanography, Dona Paula, Goa, India | |