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dc.contributor.authorPrasannaKumar, C.
dc.contributor.authorRethinavelu, S.
dc.contributor.authorSadaiappan, B.
dc.date.accessioned2020-12-31
dc.date.accessioned2021-02-09T10:13:11Z
dc.date.available2020-10-05
dc.date.available2021-02-09T10:13:11Z
dc.date.issued2020
dc.identifier.citationRegional Studies in Marine Science, vol.40; 2020; Article no: 101489
dc.identifier.issn2352-4855
dc.identifier.otherhttp://www.sciencedirect.com/science/article/pii/S2352485520306174
dc.identifier.urihttps://doi.org/https://doi.org/10.1016/j.rsma.2020.101489
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/8395
dc.descriptionIF2019 - 1.183
dc.description.abstractDue to their large size, bathynomids (Crustacea, Isopoda, Cirolanidae), belonging to the �supergiant group� of isopods, have long been prominent. The distribution of giant isopods from inshore to deep-sea environments has been reported. There was a shortage of molecular taxonomic data for these species. While DNA barcoding has proven to accelerate species identification, due to lack of reference sequences in the database, the results of environmental DNA barcoding remain mysterious. The present is therefore aimed at identifying giant isopods and generating barcode sequences for the four species that occur on India�s southeast coast. The trawl by-catches have been used for DNA extraction, amplification of COI genes and sequencing. Basic Local Alignment Search Tool (BLAST) and neighbor joining tree analysis showed that the sequences of Bathynomus kensleyi and B. decemspinosus were barcoded for the first time. DNA barcodes of B. giganteus and B. doederleinii has <3% variation compared to DNA barcodes of the same species sequenced elsewhere and made available through Genbank, whereas B. kensleyi and B. decemspinosus exhibited >7% variation to other species of the genus Bathynomus. Analysis of Kimura-2 parametric genetic distance includes 6 species of Bathynomus genera barcoded to date and an average distance of 2.1% has been found. We recommend a minimum of 3% variation based on genetic distances as a barcoding gap to differentiate and identify species in Bathynomus genera or until a new barcoding gap has been suggested after sequencing more species.
dc.languageen
dc.publisherElsevier
dc.relation.ispartofReg Stud Mar Sci
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleFirst barcodes of Bathynomus kensleyi (Lowry and Dempsey, 2006) and Bathynomus decemspinosus (Shih, 1972) from the Southeast coast of India
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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