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dc.contributor.authorNathan, V.K.
dc.contributor.authorJasna, V.
dc.contributor.authorParvathi, A.
dc.date.accessioned2020-03-31
dc.date.accessioned2021-02-09T10:13:21Z
dc.date.available2020-01-02
dc.date.available2021-02-09T10:13:21Z
dc.date.issued2020
dc.identifier.citationEnvironmental Science and Pollution Research, vol.27(4); 2020; 4468-4477
dc.identifier.issn1614-7499
dc.identifier.otherhttps://link.springer.com/article/10.1007/s11356-019-06503-1
dc.identifier.urihttps://doi.org/10.1007/s11356-019-06503-1
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/8532
dc.descriptionIF2018 - 2.914
dc.description.abstractHeterotrophic system for carbon sequestration is gaining importance in the recent decades. Carbonic anhydrase (CA) is a major enzyme involved in carbon sequestration and biomineralization process. In this paper, we evaluate the effect of pesticide on CA activity using inhibitory assay. 2,4-D, being one of the most extensively used pesticide, being deleterious to soil health, its usage should be minimized to regain the soil health. Maximum inhibitory constant (Ki) was observed for 5% 2,4-D (49.53 mM) followed by 5% glyphosate (43.92 mM). The maximum Km increase with increase in pesticide concentration by 3.05-fold was in case of glyphosate which was higher than that of 2,4-D (2.08-fold) and dichlorvos (2.38-fold). Moreover, we evaluated the carbon sequestration using CA enzyme in the soil microcosm. In the present study, we identified the negative impact of 2,4-D on carbonic anhydrase produced by Bacillus halodurans PO15. The inhibition was a mixed type and had significantly lowered the carbon reduction to about 2.38 ± 0.17% in a soil microcosm study. Based on the molecular docking, the inhibition was contributed due to weak H-bonding interaction with amino acid residues (Gly65, Gly95, Val147, Ser150 and Gly65, Ser146, and Ser150).
dc.languageen
dc.publisherSpringer
dc.relation.ispartofEnviron Sci Pollut Res
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titlePesticide application inhibit the microbial carbonic anhydrase-mediated carbon sequestration in a soil microcosm
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Kochi, Kerala, India


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