dc.contributor.author | Nathan, V.K. | |
dc.contributor.author | Jasna, V. | |
dc.contributor.author | Parvathi, A. | |
dc.date.accessioned | 2020-03-31 | |
dc.date.accessioned | 2021-02-09T10:13:21Z | |
dc.date.available | 2020-01-02 | |
dc.date.available | 2021-02-09T10:13:21Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Environmental Science and Pollution Research, vol.27(4); 2020; 4468-4477 | |
dc.identifier.issn | 1614-7499 | |
dc.identifier.other | https://link.springer.com/article/10.1007/s11356-019-06503-1 | |
dc.identifier.uri | https://doi.org/10.1007/s11356-019-06503-1 | |
dc.identifier.uri | http://drs.nio.org/drs/handle/2264/8532 | |
dc.description | IF2018 - 2.914 | |
dc.description.abstract | Heterotrophic system for carbon sequestration is gaining importance in the recent decades. Carbonic anhydrase (CA) is a major enzyme involved in carbon sequestration and biomineralization process. In this paper, we evaluate the effect of pesticide on CA activity using inhibitory assay. 2,4-D, being one of the most extensively used pesticide, being deleterious to soil health, its usage should be minimized to regain the soil health. Maximum inhibitory constant (Ki) was observed for 5% 2,4-D (49.53 mM) followed by 5% glyphosate (43.92 mM). The maximum Km increase with increase in pesticide concentration by 3.05-fold was in case of glyphosate which was higher than that of 2,4-D (2.08-fold) and dichlorvos (2.38-fold). Moreover, we evaluated the carbon sequestration using CA enzyme in the soil microcosm. In the present study, we identified the negative impact of 2,4-D on carbonic anhydrase produced by Bacillus halodurans PO15. The inhibition was a mixed type and had significantly lowered the carbon reduction to about 2.38 ± 0.17% in a soil microcosm study. Based on the molecular docking, the inhibition was contributed due to weak H-bonding interaction with amino acid residues (Gly65, Gly95, Val147, Ser150 and Gly65, Ser146, and Ser150). | |
dc.language | en | |
dc.publisher | Springer | |
dc.relation.ispartof | Environ Sci Pollut Res | |
dc.relation.isreferencedby | SCI | |
dc.rights | Only the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations | |
dc.title | Pesticide application inhibit the microbial carbonic anhydrase-mediated carbon sequestration in a soil microcosm | |
dc.type | Journal Article | |
dc.affiliation.author | CSIR-National Institute of Oceanography, Kochi, Kerala, India | |