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dc.contributor.authorAparna, S.
dc.contributor.authorParvathi, A.
dc.contributor.authorArya, K.
dc.date.accessioned2020-03-31
dc.date.accessioned2021-02-09T10:13:21Z
dc.date.available2020-01-03
dc.date.available2021-02-09T10:13:21Z
dc.date.issued2020
dc.identifier.citationEnvironmental Monitoring and Assessment, vol.192(3); 2020; Article no: 199
dc.identifier.issn1573-2959
dc.identifier.otherhttps://doi.org/10.1007/s10661-020-8166-9
dc.identifier.urihttps://doi.org/10.1007/s10661-020-8166-9
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/8537
dc.descriptionIF2018 - 1.959
dc.description.abstractMarinobacter is an ecologically important genus of Gammaproteobacteria found in diverse marine habitats, many species of which are capable of degrading hydrocarbons. In this study, we isolated a Marinobacter phage-host system from the surface waters of the Arabian Sea using enrichment culture methods, studied their growth characteristics and investigated the effect of salinity and nitrate concentrations on phage-host interactions. The bacterial isolate had maximum identity to Marinobacter salsuginis based on 16S rRNA similarities and was termed as Marinobacter sp., strain D1S9. It could tolerate up to 14% of NaCl with maximum growth at 11% NaCl. The host grew optimally between 35 and 40 °C and at pH 8. It had a generation time of 3.7 h with a mean growth rate of 0.27 h-1. The phage infected the host forming clear, round plaques of 1-2 mm diameter. It had a narrow host range restricted to the strain Marinobacter D1S9. The latent period and burst size of the phage were estimated to be 30 min and 106 phages per infected cell, respectively. The phage had an adsorption rate of 3.4 x 10-8 ml min-1 and retained 40.4% of its adsorption efficiency at 16% NaCl with a maximum at 4% NaCl (76.1%). Inorganic nitrate was found to have a direct role in controlling host growth and phage burst size.
dc.languageen
dc.publisherSpringer
dc.relation.ispartofEnviron Monit Assess
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleIsolation and characterization of a moderately halophilic Marinobacter phage-host system from the Arabian Sea
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Kochi, Kerala, India


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