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dc.contributor.authorTilvi, S.
dc.contributor.authorMajik, M.
dc.contributor.authorNaik, C.G.
dc.date.accessioned2008-02-22T04:36:13Z
dc.date.available2008-02-22T04:36:13Z
dc.date.issued2005
dc.identifier.citationEuropean journal of Mass Spectrometry, Vol.11; 345-351p.
dc.identifier.urihttp://drs.nio.org/drs/handle/2264/872
dc.description.abstractCeramides are important intracellular second messengers that play a role in the regulation of cell growth, differentiation and programmed cell death. Analysis of these second messengers requires a sensitive and specific analytical method to detect individual ceramide species and to differentiate between them. Eight molecular species of ceramide were identified from the marine sponge Haliclona cribricutis using electrospray ionization tandem mass spectrometry (ESI-MS/MS). From this marine sponge, N-hencicosanoyl (N21:0) to N-hexasanoyl (N26:0) octadecasphing-4 (E)-enine have been reported for the first time. The ESI-MS spectra gave several strong protonated (M + H) sup(+) molecular ions with the corresponding bis (2-ethyl hexyl) phthalate adduct [M+H+DHEP] sup(+). The collision-induced dissociation on ceramides at m/z 622.7337, 636.7645, 650.7789, 664.7925 and 678.8130 conducted at low-collision energy produced product ions with good characteristic at m/z 252.31, 264.32, 278.33 and 296.35 for d18:1 sphingosine, regardless of the length of the fatty chain. The MS/MS of the phthalate adduct [M+H+DHEP] sup(+) at m/z 1013.1820, 1027.1971, 1041.2176, 1055.2394 and 1069.2573 also yielded characterizing product ions for sphingosine and confirmed the molecular ion at m/z 391 for bis (2-ethyl hexyl) phthalate. The major ions in the [M+H+DHEP] sup(+) were due to neutral loss of [M+H-H sub(2) O] sup(+) and [M+H-H sub(2) O] sup(+)
dc.language.isoen
dc.publisherIM Publications
dc.rightsCopyright [2005]. It is tried to respect the rights of the copyright holders to the best of the knowledge. If it is brought to our notice by copyright holder that the rights are voilated then the item would be withdrawn.
dc.subjectmarine invertebrates
dc.subjectspectroscopic techniques
dc.subjectmass spectroscopy
dc.subjections
dc.subjectmolecular structure
dc.subjectchromatographic techniques
dc.subjectinfrared spectroscopy
dc.subjectHaliclona cribricutis
dc.titleA tendem mass spectrometric approach for determining the structure of molecular species of ceramide in the marine sponge, Haliclona cribricutis
dc.typeJournal Article


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