• Login
    View Item 
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Bioluminescence of green Noctiluca scintillans from the coastal waters of Kochi (southwest coast of India) and in vivo experiments on bioluminescence in response to mechanical stimuli and its diurnal variations

    Thumbnail
    Date
    2021
    Author
    Parvathi, A.
    Vishal, C.R.
    Madhu, N.V.
    Anil, P.
    Iqbal, P.M.M.
    Gopika, P.
    Camey, M.F.
    Metadata
    Show full item record
    Abstract
    A bloom of green Noctiluca scintillans (GNS) was spotted as conspicuous bright blue coloured bioluminescence in the coastal waters of Kochi on 29 May 2019. The intense blue colouration was visible in the inshore waters, which decreased towards offshore waters. We conducted experiments to elucidate the response of N. scintillans to mechanical stimuli and its diurnal variations (photo-inhibition and circadian rhythm) through in vivo experiments. The bioluminescence was measured using a Luminometer in three modes of speed frequencies (low, medium and high). The results indicated that the bioluminescence increased with increase in cell abundance and mechanical stimuli with an emission of 5.3 � 1.9 � 103 Relative Light Units (RLU) at high levels of mechanical stimuli. To understand the diurnal fluctuations and circadian rhythm in N. scintillans, 20 l samples were maintained in light-exposed (natural day/night) and light-deprived conditions in duplicates for 24 h. Bioluminescence was measured at 3 hourly intervals during the photophase (6:00 h to 18:00 h) and scotophase (18:00 h to 6:00 h) in Luminometer. Results suggest that the light-exposed samples exhibited peak bioluminescence (4.6 � 0.5 � 103 RLU) during the photophase (at 15:00 h when the Photosynthetically Active Radiation was 810 �mol m-2 s-1). Therefore, bioluminescence was not photo-inhibited in green strains of N. scintillans. Contrary to the earlier studies, maximum bioluminescence in light-exposed samples was lower than light-deprived samples during the photophase/scotophase. These results suggest that though light influences bioluminescence, it does not regulate its endogenous circadian rhythm in bioluminescence in green N. scintillans
    URI
    https://doi.org/10.1080/17451000.2021.2012578
    https://drs.nio.org/drs/handle/2264/8791
    Collections
    • Scholarly literature [9074]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV