Show simple item record

dc.contributor.authorJasna, V.
dc.contributor.authorParvathi, A.
dc.contributor.authorNathan, V.K.
dc.date.accessioned2022-04-01
dc.date.accessioned2022-06-20T10:44:04Z
dc.date.available2022-01-01
dc.date.available2022-06-20T10:44:04Z
dc.date.issued2022
dc.identifier.citationEnvironmental Science and Pollution Research, vol.29(3); 2022; 3967-3982
dc.identifier.issn1614-7499
dc.identifier.otherhttps://link.springer.com/article/10.1007/s11356-021-15772-8
dc.identifier.urihttps://doi.org/10.1007/S11356-021-15772-8/TABLES/4
dc.identifier.urihttps://drs.nio.org/drs/handle/2264/8816
dc.description4.223
dc.description.abstractMud banks and upwelling are two important oceanographic features occurring along the southwest coast of India during the southwest monsoon period. The study region, Alappuzha lying on the southwest coast of India, is unique due to the co-existence of upwelling and mud banks during the monsoon (MON) season. Water samples were collected from three stations, M1, M2, and M3, from April to September 2014, at weekly/biweekly intervals to determine the total bacterial abundance, viable prokaryotic counts, and total plate counts, along with measurements on physico-chemical parameters. For determining the heterotrophic culturable bacterial diversity, water samples were collected during two seasons, monsoon and pre-monsoon (PRM), from three stations. Water samples were inoculated into two non-selective broths for enrichment, DNA was extracted, and next-generation sequencing analysis was performed using Illumina Miseq sequencing. The sequence analysis revealed that dominant communities were Proteobacteria, followed by Firmicutes and Fusobacteria. Proportions of Fusobacteria increased during monsoon and proportions of Firmicutes were high in premonsoon season. Among Proteobacteria, Gammaproteobacteri is presented more than 99% of all the classes, irrespective of seasons. Vibrio was the most dominant genus during both seasons. The presence of anaerobic genera such as Propionigenium and Cetobacterium at all the stations during MON indicated the presence of upwelled waters. The genus Stenotrophomonas was observed in the M2 station alone. This study provides an overview of the culturable heterotrophic bacterial communities in a region in the southeastern Arabian Sea with coexisting mud banks and upwelling. The results of this study were compared with a published report on culture-independent bacterial diversity (from environmental DNA) from the same region. The study demonstrates that the use of culture media underrepresented the phylogenetic diversity and selectively enriched the class Gammaproteobacteria alone
dc.languageen
dc.publisherSpringer
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleDiversity pattern of marine culturable heterotrophic bacteria in a region with coexisting upwelling and mud banks in the southeastern Arabian Sea
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Kochi, Kerala, India


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record