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dc.contributor.authorBhatawadekar, V.C.
dc.contributor.authorDamare, S.R.
dc.contributor.authorGarg, A.
dc.date.accessioned2023-06-20
dc.date.accessioned2023-07-05T10:21:00Z
dc.date.available2023-05-02
dc.date.available2023-07-05T10:21:00Z
dc.date.issued2023
dc.identifier.citation3 Biotech, vol.13; 2023; Article no: 144
dc.identifier.issn2190-5738
dc.identifier.otherhttps://link.springer.com/article/10.1007/s13205-023-03549-4
dc.identifier.urihttps://doi.org/10.1007/s13205-023-03549-4
dc.identifier.urihttp://drs.nio.res.in/drs/handle/2264/9015
dc.description2.893
dc.description.abstractBioremediation using microbes is an eco-friendly approach being explored for reclaiming PAH-contaminated areas. However, isolation and screening of potential bacteria to degrade PAHs are very laborious and cumbersome. To alleviate this issue, we describe a rapid method for screening the bacterial cultures for their ability to degrade PAHs using Folin-Ciocalteu (FC) assay. Six hundred bacterial isolates were tested for their ability to degrade PAH using FC assay. The cultures capable of degrading PAH show blue colouration, resulting from the reaction of FC reagent with phenolic intermediates generated during PAH degradation. Out of the 600 cultures screened, 64 showed an ability to degrade PAH. This study provides a very easy, rapid, less laborious, and sensitive method to screen a large number of bacterial cultures for their ability to degrade PAH
dc.languageen
dc.publisherSpringer
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleFolin-Ciocalteu assay as a rapid colourimetric screening method for evaluating PAH degradation abilities of heterotrophic bacteria
dc.title.alternative
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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