• Login
    View Item 
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Production of macroaggregates from dissolved exopolymeric substances (EPS) of bacterial and diatom origin

    Thumbnail
    View/Open
    FEMS_Microbiol_Ecol_53_255.pdf (463.2Kb)
    Date
    2005
    Author
    Bhaskar, P.V.
    Grossart, H.P.
    Bhosle, N.B.
    Simon, M.
    Metadata
    Show full item record
    Abstract
    Exopolymeric substance (EPS) isolated from a pure culture of the marine bacterium Marinobacter sp. and the marine diatom Skeletonema costatum (axenic) were partially purified, chemically characterized and used as dissolved organic matter (DOM) for the production of macroaggregates. The role of organic particles such as transparent exopolymeric particles (TEP) and Coomassie stained particles (CSP) in the production of macroaggregates was experimentally assessed. Three experimental rolling tanks containing sterile medium with 1) EPS, 2) EPS + live diatom cells and 3) EPS + killed bacteria and 3 control tanks without any added EPS were used for macroaggregate production. Changes in abundance and average size of macroaggregates were monitored using image analysis, whereas TEP and CSP were enumerated microscopically. In the presence of microbial EPS, macroaggregates of a size of 23 to 35 mm2 were produced. Aggregate size and abundance varied considerably with both time and source of EPS. No correlation was observed for macroaggregate size and abundance with either TEP or CSP. One-way ANOVA demonstrated significant differences in the variance of particle abundance and size in tanks having only EPS or in combination with living diatom cells. Our data suggest that production of macroaggregates was influenced by polymer chemistry and surface properties of colliding particles whereas TEP and CSP concentrations were influenced by molecular weight of EPS and the presence of growing cells. Interestingly, macroaggregates were formed in the near absence of TEP and CSP, highlighting the role of other unknown processes in the transformation of DOM to particulate organic matter (POM) in aquatic environments.
    URI
    http://drs.nio.org/drs/handle/2264/91
    Collections
    • Scholarly literature [9074]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV