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dc.contributor.authorSneha, K.G.
dc.contributor.authorPillai, Devika
dc.contributor.authorDevika, Raj
dc.date.accessioned2023-12-18
dc.date.accessioned2023-12-11T04:44:37Z
dc.date.available2023-11-13
dc.date.available2023-12-11T04:44:37Z
dc.date.issued2023
dc.identifier.citation3 Biotech, vol.13(12); 2023; Article no: 394
dc.identifier.issn2190-5738
dc.identifier.otherhttps://link.springer.com/article/10.1007/s13205-023-03820-8
dc.identifier.urihttps://doi.org/10.1007/S13205-023-03820-8
dc.identifier.urihttp://drs.nio.res.in/drs/handle/2264/9153
dc.descriptionIF(JCR2023) = 2.8
dc.description.abstractThe emergence of microorganisms with resistance toward antibiotics has been widely recognized as a growing hazard to public health. Here, we report the whole genome sequence of Klebsiella pneumoniae strain THK, which was isolated from an aquaculture farm in Kerala, India. Standard disc diffusion and strip methods were used for antibiotic susceptibility testing and minimum inhibitory concentration detection. The isolate showed high level of resistance against beta-lactam, aminoglycosides, fluoroquinolones, macrolides, tetracyclines, chloramphenicol, sulfonamides, and cephalosporin class of antibiotics, but were sensitive to carbapenem and polymyxins. The whole genome sequence analysis revealed that the final assembly of THK strain consisted of 30 contigs of 5, 199,186 bp including four plasmid sequences plasmid_690 (1 contig), plasmid_710 (9 contigs), plasmid_1222 (6 contigs), and plasmid_1528 (1 contig). Sequence analysis revealed that the isolate has a number of genes encoding for antibiotic resistance and virulence. The findings indicated that antibiotics present in aquatic environments at sub-inhibitory concentrations increase the selection pressure affecting the cell function of even normal aquatic microorganisms to change the genetic expression of virulence factors or acquire resistance genes through various transfer mechanisms. This strain would be of significant interest for thorough comparative genomic study, given the known vast diversity of pathogenic and antibiotic resistance potentials.
dc.languageen
dc.publisherSpringer
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleComplete genome sequence analysis of multi-drug resistant Klebsiella pneumoniae THK strain isolated from aquaculture farm in Kerala, India
dc.typeJournal Article
dc.affiliation.authorDepartment of Aquatic Animal Health Management, Kerala University of Fisheries and Ocean Studies, Panangad, Kochi, Kerala, India


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