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dc.contributor.authorJagtap, A.S.
dc.contributor.authorManohar, C.S.
dc.contributor.authorKadam, N.S.
dc.date.accessioned2024-03-31
dc.date.accessioned2024-09-13T05:46:49Z
dc.date.available2024-01-16
dc.date.available2024-09-13T05:46:49Z
dc.date.issued2024
dc.identifier.citationProcess Biochemistry, vol.137; 2024; 187-196
dc.identifier.issn1359-5113
dc.identifier.otherhttps://www.sciencedirect.com/science/article/abs/pii/S1359511324000096
dc.identifier.urihttps://doi.org/10.1016/J.PROCBIO.2024.01.007
dc.identifier.urihttp://drs.nio.res.in/drs/handle/2264/9232
dc.descriptionIF(JCR2023) = 4.4
dc.description.abstractMacroalgae associated bacteria are the major source of polysaccharide hydrolyzing enzymes for efficient bioutilisation of macroalgal biomass. In this study, extracellular porphyranase, PB1 isolated from a macroalgae associated bacterium, Bacillus sp. NIOA284 was purified and characterized for hydrolysis of porphyran into porphyran oligosaccharides. The enzyme, PB1 showed the highest activity at the optimum conditions; the temperature of 35 oC, pH 7 and 0.4 NaCl with 10 mM CoCl2. The structural characterization of the enzymatically produced POS using NMR spectroscopy and HRMS spectrometry showed that the enzyme acted on porphyran as ?-porphyranase and produced di, tri and tetra saccharides. The bioactive potential of POS was determined based on its radical scavenging and prebiotic activity. Results show that POS exhibited higher antioxidant activity than parent porphyran and fructooligosaccharides. It was also capable to proliferate the beneficial gut bacterium Lactobacillus acidophilus and L. bulgaricus, it recorded a prebiotic activity score equivalent to model prebiotic compound fructooligosaccharides. Results show that the enzymatically produced POS has combined antioxidant and prebiotic activity. This highlights the significance of macroalgae associated bacteria as a source of enzymes capable of hydrolyzing polysaccharides into simple bioactive oligosaccharides for efficient management of chronic disorders caused due to oxidative stress.
dc.languageen
dc.publisherElsevier
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleCharacterization of bioactive porphyran oligosaccharides produced using porphyranase from Bacillus sp. NIOA284
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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