Vibrio harveyi modulated gene expression in Penaeus monodon and Fenneropenaeus indicus

Abstract

This work tries to contribute to a better understanding of the defense system in the post-larvae of P. monodon and F. indicus upon challenge with a virulent strain of V. harveyi. This study involved the use of SSH for construction of subtracted cDNA libraries in order to identify individual genes expressed in the post-larvae as a result of the challenge. Through qPCR analysis, gene expression profiles of different immune related genes in the post-larvae of P. monodon and F. indicus were studied. Furthermore, this study also included full length cloning and characterization of two important immune transcripts: ferritin and translationally controlled tumor protein (TCTP) from the Indian shrimp F. indicus.