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dc.contributor.authorRam, V.P.
dc.contributor.authorYasur, J.
dc.contributor.authorAbishad, P.
dc.contributor.authorRamesh, Ch.
dc.contributor.authorGourkhede, D.P.
dc.contributor.authorArya, P.R.
dc.contributor.authorVarsha, Unni
dc.contributor.authorVergis, J.
dc.contributor.authorMalik, S.V.S.
dc.contributor.authorKaore, M.
dc.contributor.authorKurkure, N.V.
dc.contributor.authorKullaiah, B.
dc.contributor.authorBarbuddhe, S.B.
dc.contributor.authorRawool, D.B.
dc.date.accessioned2024-10-03
dc.date.accessioned2024-10-10T11:34:21Z
dc.date.available2023-07-12
dc.date.available2024-10-10T11:34:21Z
dc.date.issued2023
dc.identifier.citationJournal of Drug Delivery Science and Technology, vol.86; 2023; Article no: 104741
dc.identifier.issn1773-2247
dc.identifier.otherhttps://www.sciencedirect.com/science/article/abs/pii/S1773224723005932
dc.identifier.urihttps://doi.org/10.1016/J.JDDST.2023.104741
dc.identifier.urihttp://drs.nio.res.in/drs/handle/2264/9258
dc.descriptionIF2022= 5
dc.description.abstractPhytochemicals and nanoparticles (NPs) despite having strong antibacterial activity, their limited stability and safety prevent their widespread biomedical and environmental applications. Therefore, the bio-efficacy and environmental safety of both can be increased by fabricating and conjugating them using eco-friendly processes. Herein, the impact of a novel green nanomedicine, nanosilver-conjugated thymol (AgT), was evaluated against multi-drug resistant enteroaggregative Escherichia coli (MDR-EAEC) strains using in vitro and in vivo (Galleria mellonella) assays. Characterization of AgT by UV-Vis and FTIR spectroscopy demonstrated the conjugation of thymol with biogenic nanosilver with a loading capacity of 16.60 � 0.24%. Electron microscopy revealed mono-dispersed spherical morphology with a size of 15.077 � 3.34 nm. The minimum inhibitory concentration (4-16 ?g/mL) and minimum bactericidal concentration (8-16 ?g/mL) were five to six-fold times lower than thymol. The antibacterial efficacy of AgT was at par with meropenem in the in vitro time-kill assay. Moreover, AgT was tested safe against gut lactobacilli, sheep erythrocytes, RAW 264.7 and HEp-2 cell lines; stable at various high-end temperatures, pH, proteases, cationic salts, and host sera. G. mellonella larvae treated with AgT exhibited higher survival rates and safety with reduced MDR-EAEC counts. Overall, compared to thymol, AgT showed promising therapeutic potential with improved safety.
dc.languageen
dc.publisherElsevier
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleEnhanced therapeutic efficacy of biogenic nanosilver-conjugated thymol: In vitro and in vivo evaluation against emerging multi-drug resistant microbes
dc.typeJournal Article
dc.affiliation.authorDivision of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India


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