• Login
    View Item 
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    •   DSpace Home
    • Institute's contributions
    • Scholarly literature
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Xylanase of�Bacillus�sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential

    Thumbnail
    Date
    2025
    Author
    Parab, P.D.
    Khandeparker, R.D.
    Metadata
    Show full item record
    Abstract
    Microbial xylanases are xylan hydrolyzing enzymes which has congregated attention due to their immense potential in many industries. The current study is focused on xylanase from bacterial isolate identified as�Bacillus�sp. NIORKP76 on an inexpensive agro-industrial waste (wheat bran) under solid-state fermentation. The isolate showed maximum xylanase production in growth medium supplemented with phosphate, NaCl and NH4Cl at 64?mM, 15?mg/mL and 0.3?mg/mL respectively which was maintained at 1:3 substrate to moisture ratio (w/v). Using optimized conditions, the maximum xylanase production in wheat bran was achieved in 72?h at room temperature 28?�?2?�C. Xylanase exhibited pH optimum of 8.0 and retained 92% of its residual activity after 24?h incubation at pH 8. Xylanase displayed optimum activity at 60?�C, whereas it remained unhindered even after 12?h of incubation period at 30 and 40?�C. The xylanase isolated in this study was purified to homogeneity with a molecular weight of approximately 28?kDa. Xylanase from�Bacillus�sp. NIORKP76 was potentially converting agro-residues into biofuel feedstock. Pretreated wheat bran produced the highest yield fermentable sugars (141?mg/g), after 8?h incubation with 5?U/g xylanase and at 40?�C.
    URI
    https://doi.org/10.1080/10826068.2025.2457545
    http://drs.nio.res.in/drs/handle/2264/9524
    Collections
    • Scholarly literature [9179]

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    Theme by 
    Atmire NV