| dc.contributor.author | Parab, P.D. | |
| dc.contributor.author | Khandeparker, R.D. | |
| dc.date.accessioned | 2025-09-30 | |
| dc.date.accessioned | 2025-09-30T04:44:06Z | |
| dc.date.available | 2025-02-01 | |
| dc.date.available | 2025-09-30T04:44:06Z | |
| dc.date.issued | 2025 | |
| dc.identifier.citation | Preparative Biochemistry & Biotechnology,vol.55(7);2025; PP: 835-845 | |
| dc.identifier.issn | 1082-6068 | |
| dc.identifier.other | https://www.tandfonline.com/doi/full/10.1080/10826068.2025.2457545?scroll=top&needAccess=true#abstract | |
| dc.identifier.uri | https://doi.org/10.1080/10826068.2025.2457545 | |
| dc.identifier.uri | http://drs.nio.res.in/drs/handle/2264/9524 | |
| dc.description | IF2024= 1.9 | |
| dc.description.abstract | Microbial xylanases are xylan hydrolyzing enzymes which has congregated attention due to their immense potential in many industries. The current study is focused on xylanase from bacterial isolate identified as�Bacillus�sp. NIORKP76 on an inexpensive agro-industrial waste (wheat bran) under solid-state fermentation. The isolate showed maximum xylanase production in growth medium supplemented with phosphate, NaCl and NH4Cl at 64?mM, 15?mg/mL and 0.3?mg/mL respectively which was maintained at 1:3 substrate to moisture ratio (w/v). Using optimized conditions, the maximum xylanase production in wheat bran was achieved in 72?h at room temperature 28?�?2?�C. Xylanase exhibited pH optimum of 8.0 and retained 92% of its residual activity after 24?h incubation at pH 8. Xylanase displayed optimum activity at 60?�C, whereas it remained unhindered even after 12?h of incubation period at 30 and 40?�C. The xylanase isolated in this study was purified to homogeneity with a molecular weight of approximately 28?kDa. Xylanase from�Bacillus�sp. NIORKP76 was potentially converting agro-residues into biofuel feedstock. Pretreated wheat bran produced the highest yield fermentable sugars (141?mg/g), after 8?h incubation with 5?U/g xylanase and at 40?�C. | |
| dc.language | en | |
| dc.publisher | Taylor and Francis Group | |
| dc.relation.isreferencedby | SCI | |
| dc.rights | Only the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations | |
| dc.title | Xylanase of�Bacillus�sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential | |
| dc.type | Journal Article | |
| dc.affiliation.author | CSIR-National Institute of Oceanography, Dona Paula, Goa, India | |