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dc.contributor.authorParab, P.D.
dc.contributor.authorKhandeparker, R.D.
dc.date.accessioned2025-09-30
dc.date.accessioned2025-09-30T04:44:06Z
dc.date.available2025-02-01
dc.date.available2025-09-30T04:44:06Z
dc.date.issued2025
dc.identifier.citationPreparative Biochemistry & Biotechnology,vol.55(7);2025; PP: 835-845
dc.identifier.issn1082-6068
dc.identifier.otherhttps://www.tandfonline.com/doi/full/10.1080/10826068.2025.2457545?scroll=top&needAccess=true#abstract
dc.identifier.urihttps://doi.org/10.1080/10826068.2025.2457545
dc.identifier.urihttp://drs.nio.res.in/drs/handle/2264/9524
dc.descriptionIF2024= 1.9
dc.description.abstractMicrobial xylanases are xylan hydrolyzing enzymes which has congregated attention due to their immense potential in many industries. The current study is focused on xylanase from bacterial isolate identified as�Bacillus�sp. NIORKP76 on an inexpensive agro-industrial waste (wheat bran) under solid-state fermentation. The isolate showed maximum xylanase production in growth medium supplemented with phosphate, NaCl and NH4Cl at 64?mM, 15?mg/mL and 0.3?mg/mL respectively which was maintained at 1:3 substrate to moisture ratio (w/v). Using optimized conditions, the maximum xylanase production in wheat bran was achieved in 72?h at room temperature 28?�?2?�C. Xylanase exhibited pH optimum of 8.0 and retained 92% of its residual activity after 24?h incubation at pH 8. Xylanase displayed optimum activity at 60?�C, whereas it remained unhindered even after 12?h of incubation period at 30 and 40?�C. The xylanase isolated in this study was purified to homogeneity with a molecular weight of approximately 28?kDa. Xylanase from�Bacillus�sp. NIORKP76 was potentially converting agro-residues into biofuel feedstock. Pretreated wheat bran produced the highest yield fermentable sugars (141?mg/g), after 8?h incubation with 5?U/g xylanase and at 40?�C.
dc.languageen
dc.publisherTaylor and Francis Group
dc.relation.isreferencedbySCI
dc.rightsOnly the author version of the copright reserved papers uploaded. Contact drs@nio.org for any complaints regarding copyright violations
dc.titleXylanase of�Bacillus�sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential
dc.typeJournal Article
dc.affiliation.authorCSIR-National Institute of Oceanography, Dona Paula, Goa, India


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